PNG
IHDR sRGB eXIfMM * = > i |
2022-12-21T12:01:44
Dimensions: XY(11) x λ(1)
Camera Name: Nikon C2plus
Numerical Aperture: 1.3
Refractive Index: 1.515
Number of Picture Planes: 3
Plane #1:
Name: DRAQ5
Component Count: 1
Modality: Widefield Fluorescence, Laser Scan Confocal
Camera Settings:
{Scanner Selection}: Galvano
{Detector Selection}: DU3
{Optical Path Mode}: Manual
{First Dichroic Mirror}: 405/488/561
{First Filter Cube}: 447/60
{Second Filter Cube}: 525/50 561 LP
CH1 {Laser Wavelength}: 640.0 {Laser Power}: 7.0
{PMT HV}: 87 {PMT Offset}: 0
CH2 {Laser Wavelength}: 488.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
CH3 {Laser Wavelength}: 561.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
{Pinhole Size(um)}: 20.00
{HV LinearCorrection}: Off
{Scan Direction}: One way
{Scanner Zoom}: 1.000
{Scan Speed}: 0.125
{Channel Series Mode}: None
{Line Skip}: None
{Frame Skip}: 0
{Line Average Mode}: None
{Line Average/Integrate Count}: 0
Stim1 {Type}: Nothing
{Scan Speed}: 1
Stim2 {Type}: Nothing
{Scan Speed}: 1
Stim3 {Type}: Nothing
{Scan Speed}: 1
{Head type}: Legacy (FOV18)
Microscope Settings: Nikon Ti, FilterChanger(Turret1): 1
LightPath: L100
PFS-S, mirror: Inserted
Zoom: 1.00x
Plane #2:
Name: Fluorescein (FITC) (pH 8.0)
Component Count: 1
Modality: Widefield Fluorescence, Laser Scan Confocal
Camera Settings:
{Scanner Selection}: Galvano
{Detector Selection}: DU3
{Optical Path Mode}: Manual
{First Dichroic Mirror}: 405/488/561
{First Filter Cube}: 447/60
{Second Filter Cube}: 525/50 561 LP
CH1 {Laser Wavelength}: 640.0 {Laser Power}: 7.0
{PMT HV}: 87 {PMT Offset}: 0
CH2 {Laser Wavelength}: 488.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
CH3 {Laser Wavelength}: 561.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
{Pinhole Size(um)}: 20.00
{HV LinearCorrection}: Off
{Scan Direction}: One way
{Scanner Zoom}: 1.000
{Scan Speed}: 0.125
{Channel Series Mode}: None
{Line Skip}: None
{Frame Skip}: 0
{Line Average Mode}: None
{Line Average/Integrate Count}: 0
Stim1 {Type}: Nothing
{Scan Speed}: 1
Stim2 {Type}: Nothing
{Scan Speed}: 1
Stim3 {Type}: Nothing
{Scan Speed}: 1
{Head type}: Legacy (FOV18)
Microscope Settings: Nikon Ti, FilterChanger(Turret1): 1
LightPath: L100
PFS-S, mirror: Inserted
Zoom: 1.00x
Plane #3:
Name: TRITC
Component Count: 1
Modality: Widefield Fluorescence, Laser Scan Confocal
Camera Settings:
{Scanner Selection}: Galvano
{Detector Selection}: DU3
{Optical Path Mode}: Manual
{First Dichroic Mirror}: 405/488/561
{First Filter Cube}: 447/60
{Second Filter Cube}: 525/50 561 LP
CH1 {Laser Wavelength}: 640.0 {Laser Power}: 7.0
{PMT HV}: 87 {PMT Offset}: 0
CH2 {Laser Wavelength}: 488.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
CH3 {Laser Wavelength}: 561.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
{Pinhole Size(um)}: 20.00
{HV LinearCorrection}: Off
{Scan Direction}: One way
{Scanner Zoom}: 1.000
{Scan Speed}: 0.125
{Channel Series Mode}: None
{Line Skip}: None
{Frame Skip}: 0
{Line Average Mode}: None
{Line Average/Integrate Count}: 0
Stim1 {Type}: Nothing
{Scan Speed}: 1
Stim2 {Type}: Nothing
{Scan Speed}: 1
Stim3 {Type}: Nothing
{Scan Speed}: 1
{Head type}: Legacy (FOV18)
Microscope Settings: Nikon Ti, FilterChanger(Turret1): 1
LightPath: L100
PFS-S, mirror: Inserted
Zoom: 1.00x
iTXtXML:com.adobe.xmp
2
1
1
<?xml version="1.0" encoding="UTF-8"?>
<OME xmlns="http://www.openmicroscopy.org/Schemas/OME/2015-01" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openmicroscopy.org/Schemas/OME/2015-01 http://www.openmicroscopy.org/Schemas/OME/2015-01/ome.xsd">
<Experimenter ID="Experimenter:0" Institution="" LastName="" />
<Instrument ID="Instrument:0">
<!--this instrument tag is add because of backward compatibility with NIS elements 4.30-->
<LightSource ID="LightSource:0">
<Laser Wavelength="488" />
</LightSource>
<Objective CalibratedMagnification="2.000000" ID="Objective:0" LensNA="1.300000" NominalMagnification="2" />
</Instrument>
<Instrument ID="Instrument:1">
<LightSource ID="LightSource:1">
<Laser Wavelength="488" />
</LightSource>
<LightSource ID="LightSource:2">
<Laser Wavelength="561" />
</LightSource>
<LightSource ID="LightSource:3">
<Laser Wavelength="640" />
</LightSource>
<Detector ID="Detector:0" />
<Objective Correction="PlanFluor" ID="Objective:1" Immersion="Oil" LensNA="1.300000" Model="Plan Fluor 40x Oil DIC H N2" NominalMagnification="40" WorkingDistance="240.000000" />
<Dichroic ID="Dichroic:0" Model="???" />
</Instrument>
<Image ID="Image:0" Name="E:\DATA\Jill\Solandra\Stamen\2023_01_04_nd083-MaxIP_XY10_RGB.tif">
<AcquisitionDate>2022-12-21T12:01:44</AcquisitionDate>
<Description>Dimensions: XY(11) x λ(1)
Camera Name: Nikon C2plus
Numerical Aperture: 1.3
Refractive Index: 1.515
Number of Picture Planes: 3
Plane #1:
Name: DRAQ5
Component Count: 1
Modality: Widefield Fluorescence, Laser Scan Confocal
Camera Settings:
{Scanner Selection}: Galvano
{Detector Selection}: DU3
{Optical Path Mode}: Manual
{First Dichroic Mirror}: 405/488/561
{First Filter Cube}: 447/60
{Second Filter Cube}: 525/50 561 LP
CH1 {Laser Wavelength}: 640.0 {Laser Power}: 7.0
{PMT HV}: 87 {PMT Offset}: 0
CH2 {Laser Wavelength}: 488.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
CH3 {Laser Wavelength}: 561.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
{Pinhole Size(um)}: 20.00
{HV LinearCorrection}: Off
{Scan Direction}: One way
{Scanner Zoom}: 1.000
{Scan Speed}: 0.125
{Channel Series Mode}: None
{Line Skip}: None
{Frame Skip}: 0
{Line Average Mode}: None
{Line Average/Integrate Count}: 0
Stim1 {Type}: Nothing
{Scan Speed}: 1
Stim2 {Type}: Nothing
{Scan Speed}: 1
Stim3 {Type}: Nothing
{Scan Speed}: 1
{Head type}: Legacy (FOV18)
Microscope Settings: Nikon Ti, FilterChanger(Turret1): 1
LightPath: L100
PFS-S, mirror: Inserted
Zoom: 1.00x
Plane #2:
Name: Fluorescein (FITC) (pH 8.0)
Component Count: 1
Modality: Widefield Fluorescence, Laser Scan Confocal
Camera Settings:
{Scanner Selection}: Galvano
{Detector Selection}: DU3
{Optical Path Mode}: Manual
{First Dichroic Mirror}: 405/488/561
{First Filter Cube}: 447/60
{Second Filter Cube}: 525/50 561 LP
CH1 {Laser Wavelength}: 640.0 {Laser Power}: 7.0
{PMT HV}: 87 {PMT Offset}: 0
CH2 {Laser Wavelength}: 488.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
CH3 {Laser Wavelength}: 561.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
{Pinhole Size(um)}: 20.00
{HV LinearCorrection}: Off
{Scan Direction}: One way
{Scanner Zoom}: 1.000
{Scan Speed}: 0.125
{Channel Series Mode}: None
{Line Skip}: None
{Frame Skip}: 0
{Line Average Mode}: None
{Line Average/Integrate Count}: 0
Stim1 {Type}: Nothing
{Scan Speed}: 1
Stim2 {Type}: Nothing
{Scan Speed}: 1
Stim3 {Type}: Nothing
{Scan Speed}: 1
{Head type}: Legacy (FOV18)
Microscope Settings: Nikon Ti, FilterChanger(Turret1): 1
LightPath: L100
PFS-S, mirror: Inserted
Zoom: 1.00x
Plane #3:
Name: TRITC
Component Count: 1
Modality: Widefield Fluorescence, Laser Scan Confocal
Camera Settings:
{Scanner Selection}: Galvano
{Detector Selection}: DU3
{Optical Path Mode}: Manual
{First Dichroic Mirror}: 405/488/561
{First Filter Cube}: 447/60
{Second Filter Cube}: 525/50 561 LP
CH1 {Laser Wavelength}: 640.0 {Laser Power}: 7.0
{PMT HV}: 87 {PMT Offset}: 0
CH2 {Laser Wavelength}: 488.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
CH3 {Laser Wavelength}: 561.0 {Laser Power}: 7.0
{PMT HV}: 90 {PMT Offset}: 0
{Pinhole Size(um)}: 20.00
{HV LinearCorrection}: Off
{Scan Direction}: One way
{Scanner Zoom}: 1.000
{Scan Speed}: 0.125
{Channel Series Mode}: None
{Line Skip}: None
{Frame Skip}: 0
{Line Average Mode}: None
{Line Average/Integrate Count}: 0
Stim1 {Type}: Nothing
{Scan Speed}: 1
Stim2 {Type}: Nothing
{Scan Speed}: 1
Stim3 {Type}: Nothing
{Scan Speed}: 1
{Head type}: Legacy (FOV18)
Microscope Settings: Nikon Ti, FilterChanger(Turret1): 1
LightPath: L100
PFS-S, mirror: Inserted
Zoom: 1.00x
</Description>
<InstrumentRef ID="Instrument:1" />
<ImagingEnvironment Temperature="-1.000000" />
<Pixels DimensionOrder="XYCZT" ID="Pixels:0" PhysicalSizeX="0.310740" PhysicalSizeY="0.310740" SizeC="3" SizeT="1" SizeX="1024" SizeY="1024" SizeZ="1" Type="uint8">
<Channel ContrastMethod="Brightfield" EmissionWavelength="447" ExcitationWavelength="488" ID="Channel:0" Name="Brightfield" PinholeSize="-1.000000">
<DetectorSettings Binning="1x1" ID="Detector:0" />
</Channel>
<TiffData />
<Plane DeltaT="0.000000" DeltaTUnit="ms" ExposureTime="8127.165527" ExposureTimeUnit="ms" PositionX="0.000000" PositionY="0.000000" PositionZ="1766.599976" TheC="0" TheT="0" TheZ="0" />
</Pixels>
</Image>
</OME>
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